Courses

CRISPR Tsunami: Design and Hands-on gene editing.

This interdisciplinary course will introduce hands-on application and theory of the computational tools for gRNA design followed by experimental techniques applying these gRNAs in manipulations of immortalized mammalian cells, bacteria as well as in whole organisms (zebrafish). On the computational side cutting-edge tools for on- and off-target gRNA design for Cas9 (the most widely used CRISPR enzyme) will be covered at a level matching course participants that have a molecular biology background. On the experimental side gene editing and silencing will be performed in the different organismal contexts including (1) the human pathogen, Staphylococcus aureus involving design of constructs, transformation, and assessments of expression of targeted genes, (2) mammalian cells where the course participants will introduce mutations leading to Alzheimer’s disease to create an in vitro disease model and (3) whole vertebrate organisms (zebrafish) where the course participants will introduce knockouts, which lead to albinism.

See the full course description, deadlines and how to apply:  https://kurser.ku.dk/course/SVEK23001U

______________________________________________________________________________________________________________________

The PhD course:  "Fish as experimental animals" has not yet been advertised. It is a a 7 days course, FELASA accredited and can be found in the course catalogue shortly. The course is a a blend of online and hands-on learning. Find more information about this and coming courses here